| Title |
Time-Dependent Demineralization of Tilapia (Oreochromis niloticus) Bones Using Hydrochloric Acid for Extracellular Matrix Extraction |
|
Authors |
Nisperos, M.J., Bacosa, H., Lumancas, G., Arellano, F., Aron, J., Baclayon, L., Bantilan, Z.C., Labares, M., Jr., Bual, R. |
|
Publication date |
2023 |
|
Journal |
Biomimetics |
| Volume |
8 |
| Issue |
217 |
| Publisher |
MDPI |
| Abstract |
: Tilapia (Oreochromis niloticus) is a widely cultivated fish in tropical and subtropical regions
such as the Philippines, generating substantial waste during processing, including bones that are a
valuable source of extracellular matrix (ECM). However, the extraction of ECM from fish bones requires an essential step of demineralization. This study aimed to assess the efficiency of tilapia bone
demineralization using 0.5 N HCl at different time durations. By evaluating the residual calcium
concentration, reaction kinetics, protein content, and extracellular matrix (ECM) integrity through
histological analysis, composition assessment, and thermal analysis, the effectiveness of the process
was determined. Results revealed that after 1 h of demineralization, the calcium and protein contents were 1.10 脗卤 0.12% and 88.7 脗卤 0.58 脗碌g/mL, respectively. The study found that after 6 h, the
calcium content was almost completely removed, but the protein content was only 51.7 脗卤 1.52 脗碌g/mL
compared to 109.0 脗卤 1.0 脗碌g/mL in native bone tissue. Additionally, the demineralization reaction
followed second-order kinetics with an R2 value of 0.9964. Histological analysis using H&E staining
revealed a gradual disappearance of the basophilic components and the emergence of lacunae,
which can be a |
| Index terms / Keywords |
tilapia; fish bones; demineralization; extracellular matrix; kinetics |
| URL |
|
Mindanao State
University - Iligan Institute of Technology