| Title |
Cytotoxicity of 2-Geranylgeranyl-1,4-hydroquinone Isolated from Philippine Marine Sponge Carteriospongia sp. |
|
Authors |
Chona D. Gelani, Mylene M. Uy, Shinji Ohta, Emi Ohta |
|
Conference |
98th Annual Meeting of the Chemical Society of Japan |
| Abstract |
Bioassay-guided purification of the hexane:ethyl acetate extract of the Philippine
marine sponge, Carteriospongia sp. , resulted in the isolation of a bioactive substance which
inhibited the embryonic development of sea urchin (Hemicentrotus pulcherrimus) and
starfish (Asterina pectinifera) at the early gastrulation stage at a minimum concentration of
6.25 mcg/mL and 12.5 mcg/mL, respectively. (+)HR-ESI-FTMS gave an [M+Na]+ peak
corresponding to m/z 405.2763 which confirms the structure with the molecular weight
382.2865. The 1
H and 13C NMR spectra of the bioactive compound were identical to those of
2-geranylgeranyl-1,4-hydroquinone, a known cytotoxic metabolite from a southern
Australian deep-water marine sponge Fasciospongia sp.
As of this writing, no study has been published yet regarding the inhibitory effects of
2-geranylgeranyl-1,4-hydroquinone on the embryonic development of sea urchin and starfish.
When subjected to MTT assay, 2-geranylgeranyl-1,4-hydroquinone was found to be
low cytotoxic when tested against HepG2 (liver hepatocellular carcinoma) cell lines with
79.96 脙聜脗卤 0.67 percent cell viability at 100 mcg/mL. (positive control: 23.43 脙聜脗卤0.74 percent cell
viability at 100 mcg/mL). |
| Index terms / Keywords |
Philippine marine sponge; Carteriospongia sp.; Cytotoxicity; Biologicallyactive compounds; Embryonic Development |
Mindanao State
University - Iligan Institute of Technology