| Title |
An ex vivo culture system to study thyroid development |
|
Authors |
Anne-Sophie Delmarcelle, Mylah Villacorte, Anne-Christine Hick, Christophe E Pierreux |
|
Publication date |
2014/6/6 |
|
Journal |
JoVE (Journal of Visualized Experiments) |
| Issue |
88 |
| Pages |
e51641 |
| Publisher |
JoVE |
| Abstract |
This protocol describes dissection of mouse embryonic thyroid anlagen and the culture of explants on semiporous filters or on microscopy plastic slides. This system is ideal to study morphogenetic or differentiation events occurring during thyroid development of wild type or knockout embryos, and is amenable to gain- and loss-of-function experiments. |
| Index terms / Keywords |
thyroid, explants, anlagen |
| DOI |
doi: 10.3791/51641 |
| URL |
|
Mindanao State
University - Iligan Institute of Technology