| Title |
Leptin Gene (T117C) Polymorphism and Its Association with Milk Yield Performance in Crossbred Anglo-Nubian Goats |
|
Authors |
Carlo Stephen O. Moneva, Percival P. Sangel, Amado A. Angeles, Merlyn S. Mendioro, and Renato SA. Vega |
|
Publication date |
2022/02 |
|
Journal |
Philippine Journal of Science |
| Volume |
151 |
| Issue |
1 |
| Pages |
333-339 |
| Publisher |
Department of Science and Technology, Philippines |
| Abstract |
This study was performed to determine polymorphism in the leptin gene (T117C) and investigate
the association between genotypes and milk yield in 101 crossbred Anglo-Nubian dairy goats
from Awang, Opol, Misamis Oriental and Talay, Dumaguete City, Negros Oriental in the
Philippines. Milk yield performance data were standardized to 90 and 140 d milking periods.
The goat脙垄脗聙脗聶s hair follicles were collected to extract genomic DNA. Polymerase chain reaction脙垄脗聙脗聯
restriction fragment length polymorphism (PCR-RFLP) was the method used in genotyping
with NmuCI as the restriction enzyme. Statistical analysis was performed following the two-way
factorial (2 x 4) in a randomized complete block design (RCBD), where leptin genotypes and
parity were used as main factors and farm as the blocking factor. Two genotypes (TT and TC)
were obtained. Genotypic frequencies between TT (0.50) and TC (0.50) were evenly distributed,
while allele T (0.75) occurred more frequently than allele C (0.25) in the total population
studied. Observed heterozygosity is higher than the expected heterozygosity and the genetic
variation observed in the overall population was in Hardy-Weinberg equilibrium (p > 0.05). Milk
yield traits at 140 d milking period in crossbred Anglo-Nubian dairy goats were significantly
influenced by polymorphisms in leptin (T117C). Goats having TT genotype had superior milk
yield performance (140dADMY: 0.90L 脙聜脗卤 0.058L, 140dTMP: 124.65L 脙聜脗卤 8.51L) than those with
TC genotype (140dADMY: 0.78L 脙聜脗卤 0.051L, 140dTMP: 107.90L 脙聜脗卤 7.21L). Further studies should
be conducted in other goat breeds to validate associations of leptin polymorphism with milk
production and in other economically important traits. |
| Index terms / Keywords |
Anglo-Nubian, leptin, milk yield, PCR-RFLP, polymorphisms |
| URL |
chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/viewer.html?pdfurl=https%3A%2F%2Fphiljournalsci.dost.gov.ph%2Fimages%2Fpdf%2Fpjs_pdf%2Fvol151no1%2Fleptin_gene_polymorphism_and_its_association_with_milk_yield_.pdf&clen=797775&chunk=true |
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