| Title |
Gut bacterial community profile in Pacific white shrimp Litopenaeus vannamei following 5-aminolevulinic acid supplementation |
|
Authors |
Pedrosa-Gerasmio Ivane R; Nozaki Reiko; Kondo, Hidehiro; Hirono, Ikuo |
|
Publication date |
2020/06 |
|
Journal |
Aquaculture Research |
| Volume |
00 |
| Pages |
1-12 |
| Publisher |
Wiley |
| Abstract |
Changes in the diet have been previously reported to alter the gut bacterial profile
in several organisms, including shrimp. These shifts in microbial structure either promote
beneficial effects to the host or cause diseases. Supplementation of 5-aminolevulinic
acid (5-ALA), the precursor of tetrapyrroles, has been previously reported to
enhance shrimp's immune response. To know whether 5-ALA has effects on the gut
bacterial structure in Pacific white shrimp (Litopenaeus vannamei), we investigated
the bacterial communities in the intestine and stomach of L. vannamei using highthroughput
Illumina sequencing of 16S rRNA gene libraries. One week of 5-ALA
supplementation altered the bacterial community structure (beta diversity) in both
tissues, as shown by the results of multi-dimensional scaling (MDS) plots and analysis
of similarities (ANOSIM). DESeq2 analysis revealed enrichment of differentially
abundant taxa in the 5-ALA group (e.g. Enhydrobacter and Oceaniovalibus) and control
group (e.g. Tenacibaculum and Mycobacterium). Metagenomic predictions suggested
that the control group had more KEGG pathways associated with 脙聝脗垄脙聜脗聙脙聜脗聵metabolism脙聝脗垄脙聜脗聙脙聜脗聶 than
the 5-ALA group. This study suggests that 5-ALA supplementation potentially promotes
the formation of a beneficial bacterial community structure in shrimp. This is
the first report on the effect of 5-ALA supplementation on the bacterial community
profile in any organism. |
| Index terms / Keywords |
5-aminolevulinic acid, 16S rRNA gene, gut microbiome, high-throughput sequencing, Litopenaeus vannamei |
| DOI |
|
| URL |
|
Mindanao State
University - Iligan Institute of Technology